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rp11 265b8 probe  (Vector Laboratories)


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    Structured Review

    Vector Laboratories rp11 265b8 probe
    ( a ) Two TaqMan copy number assays were applied; Hs05092416-cn assay, represented in black, was designed to amplify a 106 bp fragment of FXN within intron 3 and Hs02407730-cn assay, represented in grey, was designed to amplify an 80 bp fragment of FXN within intron 1 and exon 2. Wild type (WT) served as a negative control with no copy number. Error bars = SD. n = 2. ( b and c ) Determination of the integration site of the transgenic FXN gene by FISH. Biotin-labelled <t>RP11-265B8</t> and digoxigenin- labelled RP11-876N18 probes were hybridised onto interphase and metaphase chromosomes (DAPI stained) of YG8R, YG22R and Y47R cells. ( b ) All three cell lines showed a single integration site of the FXN transgene by metaphase analysis. ( c ) YG8R showed three hybridisation signals corresponded to the transgenic FXN , whereas YG22R and Y47R showed one signal indicating one copy of the FXN transgene. Scale bare = 10 µm.
    Rp11 265b8 Probe, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1537 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rp11 265b8 probe/product/Vector Laboratories
    Average 96 stars, based on 1537 article reviews
    rp11 265b8 probe - by Bioz Stars, 2026-05
    96/100 stars

    Images

    1) Product Images from "Cellular, Molecular and Functional Characterisation of YAC Transgenic Mouse Models of Friedreich Ataxia"

    Article Title: Cellular, Molecular and Functional Characterisation of YAC Transgenic Mouse Models of Friedreich Ataxia

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0107416

    ( a ) Two TaqMan copy number assays were applied; Hs05092416-cn assay, represented in black, was designed to amplify a 106 bp fragment of FXN within intron 3 and Hs02407730-cn assay, represented in grey, was designed to amplify an 80 bp fragment of FXN within intron 1 and exon 2. Wild type (WT) served as a negative control with no copy number. Error bars = SD. n = 2. ( b and c ) Determination of the integration site of the transgenic FXN gene by FISH. Biotin-labelled RP11-265B8 and digoxigenin- labelled RP11-876N18 probes were hybridised onto interphase and metaphase chromosomes (DAPI stained) of YG8R, YG22R and Y47R cells. ( b ) All three cell lines showed a single integration site of the FXN transgene by metaphase analysis. ( c ) YG8R showed three hybridisation signals corresponded to the transgenic FXN , whereas YG22R and Y47R showed one signal indicating one copy of the FXN transgene. Scale bare = 10 µm.
    Figure Legend Snippet: ( a ) Two TaqMan copy number assays were applied; Hs05092416-cn assay, represented in black, was designed to amplify a 106 bp fragment of FXN within intron 3 and Hs02407730-cn assay, represented in grey, was designed to amplify an 80 bp fragment of FXN within intron 1 and exon 2. Wild type (WT) served as a negative control with no copy number. Error bars = SD. n = 2. ( b and c ) Determination of the integration site of the transgenic FXN gene by FISH. Biotin-labelled RP11-265B8 and digoxigenin- labelled RP11-876N18 probes were hybridised onto interphase and metaphase chromosomes (DAPI stained) of YG8R, YG22R and Y47R cells. ( b ) All three cell lines showed a single integration site of the FXN transgene by metaphase analysis. ( c ) YG8R showed three hybridisation signals corresponded to the transgenic FXN , whereas YG22R and Y47R showed one signal indicating one copy of the FXN transgene. Scale bare = 10 µm.

    Techniques Used: Negative Control, Transgenic Assay, Staining, Hybridization



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    rp11 265b8 probe  (Vector Laboratories)
    96
    Vector Laboratories rp11 265b8 probe
    ( a ) Two TaqMan copy number assays were applied; Hs05092416-cn assay, represented in black, was designed to amplify a 106 bp fragment of FXN within intron 3 and Hs02407730-cn assay, represented in grey, was designed to amplify an 80 bp fragment of FXN within intron 1 and exon 2. Wild type (WT) served as a negative control with no copy number. Error bars = SD. n = 2. ( b and c ) Determination of the integration site of the transgenic FXN gene by FISH. Biotin-labelled <t>RP11-265B8</t> and digoxigenin- labelled RP11-876N18 probes were hybridised onto interphase and metaphase chromosomes (DAPI stained) of YG8R, YG22R and Y47R cells. ( b ) All three cell lines showed a single integration site of the FXN transgene by metaphase analysis. ( c ) YG8R showed three hybridisation signals corresponded to the transgenic FXN , whereas YG22R and Y47R showed one signal indicating one copy of the FXN transgene. Scale bare = 10 µm.
    Rp11 265b8 Probe, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rp11 265b8 probe/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    rp11 265b8 probe - by Bioz Stars, 2026-05
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a ) Two TaqMan copy number assays were applied; Hs05092416-cn assay, represented in black, was designed to amplify a 106 bp fragment of FXN within intron 3 and Hs02407730-cn assay, represented in grey, was designed to amplify an 80 bp fragment of FXN within intron 1 and exon 2. Wild type (WT) served as a negative control with no copy number. Error bars = SD. n = 2. ( b and c ) Determination of the integration site of the transgenic FXN gene by FISH. Biotin-labelled RP11-265B8 and digoxigenin- labelled RP11-876N18 probes were hybridised onto interphase and metaphase chromosomes (DAPI stained) of YG8R, YG22R and Y47R cells. ( b ) All three cell lines showed a single integration site of the FXN transgene by metaphase analysis. ( c ) YG8R showed three hybridisation signals corresponded to the transgenic FXN , whereas YG22R and Y47R showed one signal indicating one copy of the FXN transgene. Scale bare = 10 µm.

    Journal: PLoS ONE

    Article Title: Cellular, Molecular and Functional Characterisation of YAC Transgenic Mouse Models of Friedreich Ataxia

    doi: 10.1371/journal.pone.0107416

    Figure Lengend Snippet: ( a ) Two TaqMan copy number assays were applied; Hs05092416-cn assay, represented in black, was designed to amplify a 106 bp fragment of FXN within intron 3 and Hs02407730-cn assay, represented in grey, was designed to amplify an 80 bp fragment of FXN within intron 1 and exon 2. Wild type (WT) served as a negative control with no copy number. Error bars = SD. n = 2. ( b and c ) Determination of the integration site of the transgenic FXN gene by FISH. Biotin-labelled RP11-265B8 and digoxigenin- labelled RP11-876N18 probes were hybridised onto interphase and metaphase chromosomes (DAPI stained) of YG8R, YG22R and Y47R cells. ( b ) All three cell lines showed a single integration site of the FXN transgene by metaphase analysis. ( c ) YG8R showed three hybridisation signals corresponded to the transgenic FXN , whereas YG22R and Y47R showed one signal indicating one copy of the FXN transgene. Scale bare = 10 µm.

    Article Snippet: The RP11-265B8 probe was detected with Avidin D-Texas Red, biotinylated anti-Avidin D and Avidin D-Texas Red (Vector Laboratories).

    Techniques: Negative Control, Transgenic Assay, Staining, Hybridization